The influence of acoustic startle probes on fear learning in humans

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Abstract

Even though human fear-conditioning involves affective learning as well as expectancy learning, most studies assess only one of the two distinct processes. Commonly used read-outs of associative fear learning are the fear-potentiated startle reflex (FPS), pupil dilation and US-expectancy ratings. FPS is thought to reflect the affective aspect of fear learning, while pupil dilation reflects a general arousal response. However, in order to measure FPS, aversively loud acoustic probes are presented during conditioning, which might in itself exert an effect on fear learning. Here we tested the effect of startle probes on fear learning by comparing brain activation (fMRI), pupil dilation and US-expectancy ratings with and without acoustic startle probes within subjects. Regardless of startle probes, fear conditioning resulted in enhanced dACC, insula and ventral striatum activation. Interaction analyses showed that startle probes diminished differential pupil dilation between CS+ and CS− due to increased pupil responses to CS−. A trend significant interaction effect was observed for US-expectancy and amygdala activation. Startle probes affect differential fear learning by impeding safety learning, as measured with pupil dilation, a read-out of the cognitive component of fear learning. However, we observed no significant effect of acoustic startle probes on other measures of fear learning.

ID 218

Authors

Michelle I. C. de Haan, Amsterdam Brain and Cognition (ABC), University of Amsterdam, Amsterdam, The Netherlands; Department of Psychiatry, Amsterdam Neuroscience, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands. Sonja van Well, Department of Clinical Psychology, University of Amsterdam, Amsterdam, The Netherlands Renée M. Visser, Medical Research Council, Cognition and Brain Sciences Unit, University of Cambridge, Cambridge, United Kingdom. H. Steven Scholte, Amsterdam Brain and Cognition (ABC), University of Amsterdam, Amsterdam, The Netherlands Guido A. van Wingen, Amsterdam Brain and Cognition (ABC), University of Amsterdam, Amsterdam, The Netherlands; Department of Psychiatry, Amsterdam Neuroscience, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands. Merel Kindt, Amsterdam Brain and Cognition (ABC), University of Amsterdam, Amsterdam, The Netherlands; Department of Clinical Psychology, University of Amsterdam, Amsterdam, The Netherlands

Year

2018

DOI of Publication

10.1038/s41598-018-32646-1

Persistent Identifier to Dataset

10.17605/OSF.IO/E8VBN

Where was the data collected?

Spinoza Center for Neuroimaging, Amsterdam, the Netherlands

How to Cite

de Haan, M. I., van Well, S., Visser, R. M., Scholte, H. S., van Wingen, G., & Kindt, M. (2025, December 9). The influence of acoustic startle probes on fear learning in humans. https://doi.org/10.17605/OSF.IO/E8VBN

Participant Information

Participant Age

Participant Sex

Experimental Group

4 experimental conditions (within subject design): - CS+ paired with acoustic startle probe - CS+ not paired with acoustic startle probe - CS- paired with acoustic startle probe - CS+ not paired with acoustic startle probe

Stimuli

Drug Administration

No

Conditioning Protocol

Differential

Instructions CS-US Contingencies

Partially instructed (whole exp)

Number of Different US

1

US Modality

electrotactile

Number of Different CS+

2

CS+ 1: Reinforcement Rate (%)

46

CS+ 2: Reinforcement Rate (%)

46

CS+ 3: Reinforcement Rate (%)

Number of Different CS-

2

CS Modality

visual

Data Collected During MRI

Yes

Physiological Measures

measured trialwise & untransformed

Skin Conductance Response

No No

Skin Conductance Level

No No

Pupil Size

Yes Yes

Fear Potentiated Startle/Startle EMG

No No

Heart Rate

No No

Ratings

US Expectancy

Yes

US Intensity Rating

No

CS Valence

No

CS Arousal

No

CS Fear

No

CS Stress

No

CS Anxiety

No

Contingency Awareness

No

Questionnaires

Anxiety Sensitivity Index (ASI)

State Trait Anxiety Inventory (STAI-S)

State Trait Anxiety Inventory (STAI-T)